MA5 Novel Methods for Bioaccumulation Assessment
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() Bioavailability and accumulation of an optimized test set of brominated flame retardants in earthworms and SPME after exposure to soil.
van der Wal, L.1, Andersson, P. L.1, Tysklind, M.1, 1 Environmental Chemistry, Umeå University, Umeå, Sweden
ABSTRACT- Brominated flame retardants (BFR) have been used ubiquitous in modern day society with pollution of our environment as a result. The focus of most investigations is on the polybrominated diphenylether (PBDE) congeners and commercial PBDE mixtures, hexabromocyclododecane (HBCD) and tetrabromobisphenol-A (TBBPA). However, the group of BFR consists of chemicals with a wide variety of chemical properties (such as molecular size, logKow and polarity). The aim of our study was to test the bioavailability of BFR in soil and to determine which properties influence their bioavailability the most. Therefore, we used a statistical design to determine an optimal test set of BFR, that covers a large chemical variation. The chemicals from this test set were spiked to OECD-soil in three concentrations reflecting background and contaminated concentrations in soil. The earthworm Eisenia andrei and solid phase microextraction fibers (SPME) were used to study uptake of these chemicals in accumulation studies up to 30 days. SPME has been used in earlier studies and proved a good predictive tool for internal concentrations of chlorinated compounds and pesticides in earthworms. The concentrations of BFR in earthworms and SPME at equilibrium as well as kinetic data will be discussed and interpreted in terms of bioavailability and physico-chemical properties.
Key words: soil, bioavailability, earthworms, SPME