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(PM245) Induction of EROD by TCDD and selected PCBs in primary hepatocytes of brown house snakes (Lamprophis fuliginosus).

Jones, P.1, Hecker, M.1, Murphy, M.1, Giesy, J.1, Hopkins, W.2, 1 Michigan State University, East Lansing, Michigan, USA2 University of Georgia, Aiken, South Carolina, USA

ABSTRACT- Primary hepatocytes were isolated from African brown house-snakes (Lamprophis fuliginosus) and exposed to different concentrations of TCDD and 4 non-ortho PCB congeners (PCB77, 81, 126, 169) under stable culture conditions for 72hrs. After termination of the exposure EROD activity was determined in the living cells. While clear dose-response curves were obtained for EROD activity in cells exposed to TCDD, no consistent dose-response relationship was observed for the PCBs. The maximum induction in TCDD exposed cells was 2-fold greater than that of the controls. In general, a relatively large variability among responses of cells from different snakes was observed with EC50s for TCDD ranging from approximately 70 to 200pg/ml media. The only PCB that caused clear induction of EROD was PCB 126, but this response was not consistent in all experiments. The applied test system represents an effective and economic way to investigate changes in MFO systems of snakes due to exposure to environmental pollutants. This method provides relevant information by combining the advantages of in vitro systems with in vivo characteristics, reducing the number of animals to be used for chemical testing. Future studies will compare effects observed in snakes with those from other species in order to determine their sensitivity to environmental chemicals of concern.

Key words: primary hepatocytes, reptiles, POPs, MFO

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