PT12 Metals and Bioaccumulation
Exhibit Hall
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(PT146) Comparison of Waterborne Copper Bioaccumulation by Two Shrimp Species, Palaemonetes pugio and Litopenaeus setiferus.

Reed, L1, 2, Dimaano, E1, 2, Siewicki, T1, 3, Jenkins, P1, Bearden, D1, 1 USDOC/NOAA/NOS/Center for Coastal Environmental Health and Biomolecular Research, Charleston, SC, USA2 University of Charleston/Masters of Environmental Studies, Charleston, SC, USA3 University of South Carolina/School of Public Health, Dept. of Environmental Health Sciences, Columbia, SC, USA

ABSTRACT- Copper, regularly measured in large national monitoring programs, has been increasingly discharged to the environment. Anthropogenic inputs of copper include industrial, agricultural, and residential sources. In urban settings, copper enters surface waters as stormwater runoff or from discharge of treated wastewater. Copper can be acutely or chronically toxic to marine life but is also an essential element. Lethal copper concentrations vary greatly among aquatic species due, in part, to their ability to regulate internal copper concentrations. Basic toxicokinetic properties of copper in the adult grass shrimp, Palaemonetes pugio, and the white shrimp, Litopenaeus setiferus were derived to be used in risk assessment modeling of estuarine contamination. Effects of acute copper exposure on grass shrimp were evaluated. To study waterborne bioaccumulation of copper, grass shrimp were exposed to 0.16 or 0.80ug/ml copper, and white shrimp were exposed to 0.16ug/ml for 14 days with static bi-weekly renewal and compared with unexposed shrimp. Tissue accumulation and distribution of copper in the white shrimp were determined during a 14 day study. At predetermined time intervals, three replicate shrimp were removed from each exposure for dissection or frozen whole at -70°C until analysis. Shrimp samples were acid digested and analyzed by ICP-MS for total copper. Whole body accumulation of copper by both species of shrimp was described with a monoexponential equation, suggesting one-compartment toxicokinetic disposition resembling constant rate intravenous infusion.

Key words: bioaccumulation, copper, Palaemonetes pugio, Litopenaeus setiferus

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