PT07 Chemical and Biological Analysis of Endocrine Disrupting Compounds
Exhibit Hall
8:00 AM - Tuesday
(PT100) Evaluation of shallow groundwater from California for estrogenic activity using Japanese medaka (Oryzias latipes).
Xie, Lingtian1, Hinton, David2, Schlenk, Daniel1, 1 Department of Environmental Sciences, University of California at Riverside, Riverside, CA, USA2 Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC, USA
ABSTRACT- For 24 days, adult male medaka were continuously exposed to shallow groundwater that originates fromrecharge of an effluent-dominated river in California in a flow-through system and control water which was initially treated with reverse osmosis, ultraviolent light and carbon filtration, then reconstituted with a similar constituency of major ions as that of the shallow groundwater. The shallow groundwater was collected from 25-70 feet below the surface of the river and had a subsurface residence time of approximately three to six months from the time it entered the subsurface . There was a significant difference (p < 0.01) in vitellogenin (vtg) levels between male medaka exposed to shallow l groundwater and those exposed to control water. Male medaka exposed to the shallow groundwater had vtg concentrations of 1086.7 +/- 324.6 ng/mg (mean +/- standard error, n=20), while control males had vtg concentrations of 55.01 +/- 13.0 ng/mg (n=20). Our results indicated the presence of estrogenic activity in the shallow groundwater derived from the effluent dominated river. Ongoing studies are in the process of determining the potential source(s) of the estrogenic activity (Supported by WERF 01-HHE-4a)
Key words: groundwater, Japanese medaka, estrogenicity, vitellogenin