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PW05 Degradates in the Environment
(PW045) Use of Short-Term Pure Culture and Sludge Batch Biodegradation Screens to Model the Fate of Pharmaceuticals in a Sewage Treatment Plant.
Ferraina, R1, Ericson, J1, Wong, J1, Scully, D1, Burns, M1, Brown, M1, Doro, A1, 1 Pfizer Global R&D, Groton, CT, USA
ABSTRACT- New active pharmaceutical ingredients (APIs)are characterized during drug development for their potential elimination via sewage treatment plants (STPs). Five-day pure culture and seven-day batch-activated sludge (BAS) screens were utilized to model the fate of APIs in STPs. The pure culture screen utilized environmental microorganisms typically found in soils and sludge; the BAS screen used activated sludge from a municipal STP. The biotransformation to metabolic intermediates was evaluated with four APIs, and compared to the results of a more extensive STP simulation test, the porous pot bioreactor. In all API tests, the pure culture biotransformation products were also observed in BAS screens. Likewise, the amount and types of biotransformation products generated during the seven-day BAS screen mimicked those observed in the porous pot bioreactor. Our results show that the five-day pure culture screen can be utilized to predict the biotransformation products of APIs expected in the environment. In addition, the seven-day BAS screen is an effective and relatively quick method to assess the biodegradation of APIs during sewage treatment, and can be utilized as a screen to define the necessity for performing longer term simulation tests such as the porous pot.
Key words: screen, biotransformation, sludge
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