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(PH211) Determination of transfer of TCE to edible parts of apple and peach trees: greenhouse study.

Parisi, R1, Doucette, W, Chard, B, Chard, J, Crouch, C, Carlson, T, Bugbee, B, Gorder, K2, 1 Utah State University, Logan, Utah, Logan2 Hill Air Force Base, Roy, Utah, U.S.

ABSTRACT- A greenhouse study was initiated in 2002 to simulate TCE exposure to trees growing above a contaminated plume adjacent to Hill Air Force Base, Utah. Dwarf apple and peach trees were irrigated with water containing TCE (0.1 and 100 ppb) via an underground drip system. Control apple and peach trees, located in the same greenhouse and offsite (to account for any potential aerial uptake), were watered with non-TCE contaminated water in the same manner. Fruit and leaf samples were collected periodically throughout the growing season and analyzed for TCE and 14C via headspace gas chromatography/ mass spectrometry (HS/GC/MS) and liquid scintillation counting (LSC), respectively. TCE-water reservoir samples were also collected weekly to determine actual TCE exposure. The plant uptake and transfer of TCE into edible fruits was compared to sulfolane, a highly water soluble, neutral organic known to be readily taken up into plants. 14C was found in all plant tissues in the TCE uptake experiments but parent TCE was found only in roots and trunk samples suggesting that the 14C is either metabolites or non-extractable TCE. This was in contrast to the results observed for the sulfolane uptake experiments where sulfolane was found in all plant tissue samples including the fruit. Sulfolane concentrations were highest in the leaves and about 100 times greater than found in the fruit.

Key words: risk assessment, plant uptake, TCE


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