WA2 Chemical and Biological Analysis of Endocrine Disrupting Compounds
255 Portland Ballroom
8:00 AM - 12:00 PM, Wednesday
() Correlation of actual exposure levels, internal dose, estrogenic activity in bile and plasma VTG levels of juvenile sea bass after exposure to bisphenol A.
Lamoree, M.H.1, Booij, P.1, Cenijn, P.H.1, Legler, J.1, Correia, A.D.2, Freitas, S.2, Henriques, M.A.2, 1 Institute for Environmental Studies, Vrije Universiteit, Amsterdam, The Netherlands2 CIIMAR Centro de Investigação Marinha e Ambiental, Porto, Portugal
ABSTRACT- Exposure of aquatic wildlife in surface waters to (xeno-)estrogens is known to cause reproductive dysfunction. As part of a study on the combination effects of mixtures of estrogenic chemicals in marine and freshwater organisms (ACE, EVK1-CT-2001-100), screening studies on vitellogenin (VTG) induction in juvenile sea bass (Dicentrarchus labrax) were carried out after exposure to bisphenol A (BPA). Juvenile sea bass were exposed to various concentrations of BPA using a flow through system. Fish were exposed for a period of two weeks, at the end of which blood and bile samples were collected. The plasma was assayed for VTG concentration using a sea bass-VTG ELISA. Actual BPA concentrations in the water in the exposure tanks were determined by HPLC coupled to diode array detection. BPA levels in fish bile, representing the internal dose, were determined according to a method presented earlier by Houtman et al. (13th Annual Meeting SETAC Europe, 2003). After deglucuronidation, bile samples were extracted and fractionated by reversed phase HPLC. BPA was derivatized prior to quantitation using GC with ion trap detection. Unfractionated and fractionated bile extracts were also tested for estrogenic response in the ER-CALUX® assay. BPA concentrations in bile were correlated to actual exposure concentrations, estrogenic activity of bile in the ER-CALUX® assay and to plasma VTG levels.
Key words: ER-CALUX®, bile, vitellogenin, bisphenol A