PH05 Fate and Effects of Energetic Compounds
Exhibit Hall
8:00 AM - Thursday
(PH030) Risk assessment of the TNT-contaminated soil material Sickergraben by biotests with terrestrial invertebrates.
Schaefer, Dr. R.K.1, Achazi, Prof. Dr. R.K.1, Tix, A.1, Höhne, P.1, Warnecke, Dr. D.1, 1 FU-Berlin, Insitute of Ecotoxicology and Biochemistry, Germany
ABSTRACT- Risk assessment of field soils, contaminated soil and soil material obtained by remediation techniques, cannot be based on the chemical-analytical determination of the total contents of specific contaminants, because they do not provide information of either unexpected substances and of their bioavailable fractions. To assess the mobility and bioavailability of contaminants and their uptake and metabolisation by micro-organisms, plants and animals including man, biotests have to be applied. For terrestrial invertebrates standardised biotests with earthworms, collembola and enchytraeids exist. To reduce the test duration for the assessment of soil toxicity two alternative test systems were developed and applied. In the choice test enchytraeids or earthworms can choose between contaminated and uncontaminated soil material during a test period of 48 h. The effect on the reproduction of a multicellular soil invertebrate is tested with the nematode Caenorhabditis elegans after a test period of 96 hours. The three standardised tests as well as the newly developed test systems were used to asses the risk of the highly TNT-contaminated soil material Sickergraben containing 57 g TNT/kg soil (dw). The results are compatible with the one of a test battery for the retention and the habitat function of soil.
Key words: test battery, biotests, TNT, contaminated soil