MP13 Aquatic Ecotoxicology
Monday, 14 November 2005: 8:00 AM - 6:30 PM in Exhibit Hall

(CHO-1116-414827) Identification of hemoglobin in Chironomus riparius and Daphnia magna as a potential biomarker of freshwater monitoring.

LEE, S¹, LEE, S¹, HA, M¹, CHOI, J¹, ¹ University of Seoul, Faculty of Environmental Engineering, Seoul, Korea

ABSTRACT- In an attempt to identify invertebrate hemoglobin (Hb) as a biomarker of freshwater monitoring, Hb alterations were studied in two invertebrate species, Chironomus riparius and Daphnia magna, exposed to various environmental pollutants (nonylphenol, bisphenol-A, bis(2-ethylhexyl)phthalate, endosulfan, paraquat dichloride, chloropyriphos, fenitrothion, cadmium chloride, lead nitrate, potassium dichromate, benzo[a]pyrene and carbon tetrachloride). Chemical-induced Hb alterations were evaluated, in terms of gene expression by RT-PCR, total contents by cyanometHb procedure and oxidation status by multi-wavelength rapid-scanning spectrophotometry. The results suggest that Chironomus and Daphnia Hb could give useful information to diagnose freshwater quality. Considering the potential of Chironomus riparius and Daphnia magna as biomonitoring species, and the physiological particularities of their respiratory pigments, Hb in these species seems to be a promising biomarker.

Key words: Chironomus riparius, Daphnia magna, hemoglobin, biomarker