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WP1 Wastewater Treatment Effluents: Endocrine Disrupters and Pharmaceuticals
(COL-1116-988726) A Rapid Method to Quantify Estrogenic Compounds in Wastewater with Recombinant Yeast.
ABSTRACT- Many studies use recombinant yeast Saccharomyces cerevisiae with the human estrogen receptor and a reporter gene to quantify estrogenic compounds in wastewater. The usual protocol is to filter 3 or 4 liters of water through a series of filters followed by solid phase extraction. The estrogenic compounds are eluted from the solid phase extraction disk, dried, and dissolved in DMSO. The yeast is incubated in the presence of a small quantity of the concentrate, ie 1.0 microliter per ml of medium, and the response of the reporter system of the yeast is tested after incubation. We have modified the protocol so that only 50 ml of wastewater is filter sterilized with a bacteriological filter and then the yeast is grown directly in the wastewater that is diluted by half with 2X medium. The processing time of the wastewater is reduced from over 4 hours to less than 15 minutes. We have measured estradiol-equivalent concentrations down to 10 parts per trillion in wastewater by this method. The range of estradiol equivalents in influent of wastewater treatment plants near Easton, Pennsylvania ranged from less than 10 to 54 parts per trillion and in effluent from less then 10 to 20 parts per trillion.
Key words: estrogenic compounds, transgenic yeast, rapid method
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