RP5 Endocrine Disruption and Pharmaceutical Issues
Thursday, 17 November 2005: 8:00 AM - 6:30 PM in Exhibit Hall

(KIM-1117-685486) Vitellogenin Induction by Several Pharmaceuticals to Oryzias latipes.

Kang, Hee Joo¹, Kim, Kyung Tae¹, Kim, Hyun Soo¹, Kim, Pan Gyi¹, ¹ Yongin University, Yongin, Kyoggi-Do, Repulic of Korea

ABSTRACT- Endocrine disrupting effects of four pharmaceutical products were evaluated with fish. The test pharmaceuticals, sulfamethoxazole, sulfamethazine, oxytetracycline and tetracycline have been often detected in aquatic environment of Korea (from on going study of this research team). But the ecological hazard on receptors of various trophic levels has seldom been evaluated. In the present study, we conducted acute vitellogenin induction assay with a fish, Oryzias latipes. We analyzed vitellogenin induction by qualitative (Western blot) and quantitative (ELISA) assay. We used the fish groups where more than 90% individuals has plasma vitellogenin levels below the detection limit by ELISA. 17-estradiol (Sigma E-8875) was used as a positive control. E2 was dissolved in ethanol and diluted to 10ng/ml in rearing water just before use. Male and female fish were exposed to 10ng/ml E2 for 3-5 days. Aqueous solution of chemicals were exchanged every 2 days. Sulfamethoxazole, sulfamethazine, oxytetracycline and tetracycline were dissolved in ethanol and diluted 1-20ppm in rearing water just before use. Sulfamethoxazole 10, 5, 1 ppm exposure induced vitellogenin even at male fish. Sulfamethazine 10, 5, 1ppm could induced vitellogenin at male fish. Oxytetracycline 10, 5, 1ppm could induced vitellogenin with the fish. Tetracycline 20, 10, 5 ppm exposure could induced vitellogenin at male fish. Some pharmaceuticals such as sulfamethoxazole, sulfamethazine, oxytetracycline and tetracycline could give effects to male Oryzias latipes. They could induced vitellogenin under exposure from 1-20 ppm of chemicals at male Oryzias latipes.

Key words: pharmaceuticals, vitellogenin, Oryzias latipes, sulfa drugs and tetracyclines