MP7 Toxicogenomics in Environmental Studies
Monday, 14 November 2005: 8:00 AM - 5:30 PM in Exhibit Hall

(AAA-1117-816195) Development and Characterization of Japanese Medaka (Oryzias Latipes) Random cDNA microarray for Toxicogenomic Analysis.

Gu, M.B.², Park, K.S.¹, Kim, H.¹, Youn, C.H.¹, ² Graduate School of Biotechnology, Korea University, Seoul, Korea¹ National Research Laboratory on Environmental Biotechnology, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea

ABSTRACT- In our previous studies, a stress-specific functional cDNA microarray for Japanese Medaka fish had been developed by using 25 stress-related known genes, and then it had been extended for 120 stress-specific known genes. Those cDNA microarrays were used to characterize various toxic chemical impacts on fish in terms of expression profiling and trends of specific gene expression with aid of real time PCR technique. However, there have been some limit in understanding the behaviour of unknown genes and finding a new biomarkers using those functional cDNA microarrays. Therefore, in this study, random cDNA microarrays for Japanese medaka fish were developed for toxicogenomic analysis of the impacts of various chemicals or contaminants including endocrine disrupting chemicals, and other toxic chemicals. In the construction of random cDNA microarray, complete sequence information and annotation of genes are not required but may allow to have many spots of cDNAs. In this study, about 2000 random cDNA library genes were spotted on the chip. In this presentation, the construction and characterization of this chip for model chemicals will be presented.

Key words: cDNA microarray, Japanese medaka, toxicogenomic analysis, random cDNA microarray