MP7 Toxicogenomics in Environmental Studies
Monday, 14 November 2005: 8:00 AM - 5:30 PM in Exhibit Hall

(AAA-1117-817626) Toxicogenomic Analysis of EDCs (Endocrine Disrupting chemicals) Using Japanese Medaka (Oryzias Latipes) Random cDNA Microarray.

Gu, M.B.², Park, K.S.¹, Kim, H.¹, ² Graduate School of Biotechnology, Korea University, Seoul, Korea¹ National Research Laboratory on Environmental Biotechnology, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea

ABSTRACT- In order to conduct toxicogenomic analysis of Japanese Medaka (Oryzias Latipes) fish for 17-beta estradiol (E2), Nonylphenol (NP) and Phenol (P), newly fabricated random cDNA microarray containing more than 2000 random cDNAs were used. All microarray experiments were conducted with male fish. Two different concentrations of each chemical, which were derived from acute toxicity test, were exposed to fish, and liver samples obtained at different time were used for DNA microarray experiments. The results were repeatedly confirmed for biomarker genes previously known and additional expression profiling and bioinformatic analysis have revealed new findings. The results from time course expression analysis as well as self-organizing mapping (SOM) studies will be presented with biomarker analysis.

Key words: toxicogenomic analysis, Japanese Medaka, EDCs, random cDNA Microarray