MP7 Toxicogenomics in Environmental Studies
Monday, 14 November 2005: 8:00 AM - 5:30 PM in Exhibit Hall

(AAA-1117-819337) Gene Expression in Medaka Embryo and Fry Exposed to EDCs and New program for Monitoring.

HU, JY¹, Zhang, ZB¹, Sai, LL¹, Hou, YF¹, ¹ College of Environmental Science, Peking University, Beijing, Beijing, China

ABSTRACT- A number of synthetic as well as natural chemicals are capable of interfering with sex differentiation and development of wildlife, including fish, amphibians, reptiles, birds, and mammals. These so-called endocrine disrupting chemicals (EDCs) may lead to sex retarding, intersex or sex reverse. Many studies have demonstrated that the early development (including embryo and larva) of animal are the most susceptible stage to EDCs exposure. So we established the method of isochronous isolation of total RNA and genomic DNA from single embryo or fry of medaka ( Oryzias latipes ), developed method of sex indentification by genomic DNA, and investigated the differential gene expression in male or female embryo and fry exposed to different EDCs by quantitative real-time RT-PCR. And several sex differentiation related gene expressions are significantly disrupted was found. On the other hand, the in vivo experiment with embryo or fry is less expensive and with less volume than experiment with adult fish. So we will bring forward a new program for monitoring EDCs in this report.

Key words: Endocrine Disrupting Chemicals (EDCs), Gene Expression, quantitative Real-time RT-PCR, Oryzias latipes