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(T/EH032) Genotoxicity assay for polycyclic aromatic hydrocarbons in soil. Marchenko, Anatolyi1, Vorobyov, Alexey1, Pothuluri, Jairaj2, Marchenko, Sergey1, Borovick, Roman1, 1 2 ABSTRACT- For genotoxicity assay the several soil samples contaminated with coal tar, creosote and oil tar from the various regions of Russia are characterized. Pretreatment of the samples is carry out by extraction with water and organic solvents (hexane:acetone and dichloromethane). The SOS-test with Echerichia coli PQ37, Salmonella /microsome and Allium test for mitotic aberrations are performed. The solid-phase genotoxicity assay, based on mutant reversal of rifampicin-resistant Pseudomonas putida is used in parallel. The soil samples are characterized with chemical and biological methods. Gas Chromatography analysis demonstrated that aqueous extract resulted in for 24-30% recovery of solvent extracts. The data show that water-soluble mutagens resulted in for 50-60% recovery of the solvent mutagenic activity compared 30-35% recovery in solid-phase genotoxicity assay with rifampicin-resistant Pseudomonas putida. These results demonstrate that solvent extraction and solid-phase genotoxicity assay may overestimate the concentration values of genotoxic contaminant which are available for human and soil micro- and mezofauna exposure.The investigations have been performed under financial support of the ISTC, project # 1429. Key words: genotoxicity, PAH, soil, contaminant |
