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(M/EH007) Impact of Petrochemicals on the Photosynthesis of Tetraselmis chuii Using Chlorophyll Fluorescence. Pereira, Solange1, Nascimento, Iracema1, Silva, Cristiane1, Rodrigues, Anderson1, 1 ABSTRACT- Chlorophyll fluorescence has been a well-accepted diagnostic technique for the assessment of stress plants and photosynthesis rate for the last years. Acute petrochemical contamination frequently occurs in the form of oil spills, but the greater danger to marine coastal habitats is from chronic petrochemical toxicity associated with urban run-off, oil refinaries, drilling fluids, petrochemical storage and pipelines. The purpose of the present investigation is to determine the toxicity of one crude oil and a drilling fluid, to a marine microalgae using chlorophyll fluorescence. Laboratory-cultured Tetraselmis chuii were submitted to different concentrations of SPP(suspended particulate phase) of an crude oil and an drilling fluid (the Condensado Argelino base Naftenica crude oil and an drilling fluid (Sofester Drill). The preliminary results showed microalgae tolerance to petrochemical exposure up to 75% (75000 ppm) of the SPP. The parallel test with an reference toxicant the Dodecyl sodium sulfate (DSS) showed a lower tolerance of the microalgae to exposure up to 5 ppm. EC50-96h values estimated based on the final number cells, were, 10.11, 87.95, 77.01% respectively to DSS, crude oil and drilling fluid. Key words: Toxicity, Petrochemicals, Microalgae, Photosynthesis |
