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PARENT SESSION 1C Ecologically relevant and cost-effective toxicity test designs 9:00 AM to 7:00 PM, Monday, 07 May 2001
(M/EH010) Ecotoxicological characterization of hydrocarbon-contaminated soil material from an old petrol station.
Joutti, Anneli1, Schultz, Eija1, Vaajasaari, Kati2, Häkkilä, Salla1, Dorn, Susanne1, 1 2
ABSTRACT- Petroleum hydrocarbons are one of the most abundant sources of environmental contamination. Thus techniques for monitoring the environmental effect of these compounds need to be developed. A chemical characterization of these waste materials may provide information to describe the persistence of a specific compound but chemical analysis, however, cannot describe the ecotoxicological potential of a complex mixture of hydrocarbons. In our previous study we demonstrated the usefulness of several ecotoxicological tests for characterization of the compost material containing old refinery waste (A Battery of Toxicity Tests as Indicators of Decontamination in Composting Oily Wastes, Juvonen et al., Ecotoxicol. Environ. Saf., 47,156-166, 2000). We focus our present study on a hazard assessment of the oil contaminated soil from an old petrol station in contex of their landfill disposal to a non-hazardous landfill. Six samples from this hydrocarbon-contaminated soil area were tested with toxicity tests based on soil animals (Enchytraeus albidus survival and reproduction test) and plants (growth inhibition tests of onion, turnip and lettuce). Aquatic tests such as luminescent bacteria, enzyme inhibition (reverse electron transport), plant growth inhibition tests (onion), ToxiChromoPAD and mutatoxicity test MutaChromoPLATE were used for determining the toxicity of the soil water extracts. The terrestrial toxicity tests (direct soil examination) seemed to be more sensitive than the aquatic tests of the soil eluates to determine the toxicity of soil samples containing insoluble and highly volatile petroleum hydrocarbons.
Key words: hydrocarbon-contaminated soil, soil eluate, terrestrial toxicity tests, mutatoxicity
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