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(M/EH011) Physiological parameters affected by toxicity. de la Jara Valido, Adelina 1,2, Martel Quintana, Antera2, Mendoza Guzmán, Héctor1, 1 2 ABSTRACT- Toxic compounds spilling to the environment causes several effects at different organization levels. Rapid detection of these effects at the basic organization level (subcellular and cellular) should allow us to quantify the toxicity at both, low waste effluents concentrations and short exposure times, increasing, therefore, the number of processed samples and minimising costs. This work shows the results of a series of experiments carried out to assess the use of morphologic and/or physiologic related parameters like maximum photosynthesis, photosaturation intensity or cellular optical properties measurements using a Photosynthetic Light Dispensation System (Illuminova), a Plant Efficiency Analyser (Hansatech) and a FACSCalibur Flow Cytometer, in the toxicity quantification as well as in the understanding of toxicant effects on algal cells. Different degrees of sensitivity were observed on algal test organisms after 24 hours of exposure to several toxicants depending on the light / dark regime. Key words: algae, photosynthesis, toxicity-tests |
