PARENT SESSION
TA7a Bioassays for specific hazards
9:00 AM to 11:00 AM, Tuesday, 08 May 2001
Session Chair: H.T. Ratte
Room 7

(198) Free radical production in grouper (Epinephelus tauvina) exposed to xenobiotics.

Liu, Wenhua¹, Lam, Paul¹, Wu, Rudolf¹, ¹

ABSTRACT- Reactive oxygen species (ROS) are known to be produced in animals exposed to xenobiotics, UV, hypoxia and certain toxins, and these ROS can cause damage to intracellular molecules such as proteins, DNAs and membrane lipids. In this study, the grouper (Epinephelus tauvina) were exposed to menadione, benzo[a]pyrene and duroquinone, which have different free radical inducing capabilities. Free radical production induced by different xenobiotics was detected in vitro by GC-FID method,and in vivo free radical was also detected. In addition, total oxyradical scavenging capability (TOSC) and the byproducts characteristic of oxidative damages including lipid peroxide (LPO) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were also measured. The relationship between free radical production and antioxidant activities was examined, and changes in the levels of LPO and 8-OHdG were also monitored over different exposure durations.

Key words: Reactive oxygen species, TOSC, LPO, 8-OHdG