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(T/EH115) Endogenous Factors Associated with the Test Organisms Increase the Solubility of the Test Chemcial in a Regulatory Toxicity Test. Handley, John1, Mullee, Darren1, McKenzie, John1, 1 ABSTRACT- Analysis of test samples derived from ecotoxicity studies showed an increase in solubilised test chemcial due to the presence of endogenous material suspected to originate from the fish in the study. The material under test was a substituted alkane anilide with a structure consisting of polar and non-polar similar to a typical non-ionic surfactant. In pre-study in-vitro validation work with test medium (not containing fish) the recoveries of filtered water samples indicated a concentration of <1.0 mg/l (the limit of quantitation. During the in-vivo phase the test samples after 24 hours indicated an increase in concentration of 14 mg/l in filtered samples. Further post-study analysis (in-vivo and in-vitro respectively) indicated an increase in solubility due to the presence of fish and an increase in concentration when surfactants were added. The results are discussed with reference to the bioavailability and expression of toxicity to the test organisms. Key words: ecotoxicology, analysis, surfactants, bioavailability |
