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PARENT SESSION
5B The use of biomarkers for assessing ecosystem damage
9:00 AM to 7:00 PM, Wednesday, 09 May 2001

(W/EH126) Combined effect of copper and chromium on selected enzymes of the estuarine edible crab Carcinus maenas.

Elumalai, M.1, Guilhermino, Lúcia1,2, 1 2

ABSTRACT- In natural conditions, aquatic organisms are not exposed to a single chemical. Industrial and tanneries effluents contain a variety of pollutants including heavy metals. Both copper and chromium have been shown to be highly toxic to aquatic species. The estuarine edible crab has a very dynamic copper metabolism with the biosynthesis and degradation of its respiratory pigment hemocyanin. In this work, the combined effect of copper and chromium on the activity of the enzymes cholinesterase (ChE), lactate dehydrogenase (LDH) and glutathione-S-transferase (GST) of estuarine edible crab Carcinus maenas was investigated. Both individual metals significantly inhibit ChE, LDH and GST after an in vivo exposure of 96h.The mixture induced synergist effects on the enzymes studied.

Key words: Copper, Chromium, Crab Carcinus maenas, Enzymes