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(W/MF132) Adaptation of insecticide-metabolizing enzymatic activity assays in individual Frankliniella occidentalis to microscale. Cervera, Amelia1, Maymó, Ana1,2, Sarabia, Raquel1, Martínez, Rafael1, Garcerá, Mª Dolores1, 1 2 ABSTRACT- In so far as the assessment of environmental risks is concerned, it would be invaluable to be able to predict the effects of pollutants on populations and comunities on the basis of individual measures of biochemical parameters. Using insect resistance to pesticides as a marker of sensitivity to toxicant exposure this might then be taken as a population-level biomarker which could be directly correlated with known enzymatic biomarkers such as MFOs, GST or esterases. This work presents the characterisation of biochemical assays for measuring the pesticide-metabolizing enzymatic activities known to be involved in insecticide resistance mechanisms in insects. Characterisation has been performed on field samples of Frankliniella occidentalis. The final aim is to optimise microplate assays for measuring individual thrips homogenates with their subsequent measuring in a microplate reader. Key words: biomarkers, population-level, insecticide resistance, Frankliniella occidentalis |
