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(W/MF135) Measure of toxic equivalent quantities (TEQs) of PAHs in the mussel. Faucet, jérôme1, Burgeot, Thierry1, Mathieu, Michel2, Heude, Clothilde2, Maurice, Manuelle1, 1 2 ABSTRACT- Combining both chemical and toxicological data, the TEQ concept can be useful in carrying out predicative screening of environmental exposure. The main topic of this study is to develop TEQs for PAHs in a marine bivalve, Mytilus edulis, a sentinel species widely used for monitoring the quality of coastal water and showing a strong capacity for bioaccumulation of chemical contaminants. The first step in the development of TEQs for the mussel was done with primary cultures of cells. The selection of the different cellular types was made both according to cellular viability measured by the MTT test and according to the capacity for reproducing optimal conditions for maintaining the cultures. The cultures were then exposed to variable concentrations of [3H] benzo(a)pyrene (1-2000 nM), so as to estimate the incorporation of this compound in the cells. The most suitable cellular types for culturing will then be exposed to PAHs using concentrations comparables with those observed in the marine environment (10-5000 ng/g of mussel in dry weight). A suite of bioassays will then be tested on the primary cultures to determine TEFs (toxic equivalent factor) which are useful for the calculation of the TEQs. Key words: mussel, TEQs, primary/cultures, PAHs |
