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(T/EH009) Evaluation of the impact of pulp and paper mill effluents in Japan. Kishi, Katsuyuki1, Tatarazako, Norihisa1, 1 ABSTRACT- In the past several years, we have investigated the environmental impact of pulp and paper mill effluents using a battery of bioassays combined with chemical analyses. Numerous studies have revealed that the exposure to pulp and paper mill effluents alters physiological status of organisms. In this study, we describe the method to measure the P450 activities using chick embryo and fish. The enzyme activities were determined by HPLC method with high sensitivity since fluorescence background is removed. Results indicate that EROD, MROD, and PROD activities were induced in chick embryos by effluents of several mills, whereas no induction of BROD activity was detected. Field studies also revealed the presence of EROD and MROD inducers in pulp mill effluents. In contrast, the elevated PROD activity was observed in fish collected near a sewage plant. The comparison between field studies and laboratory tests and the potential use of multi-enzyme activities to characterize the origin of pollution will be discussed. Key words: pulp and paper mill effluents, P450, vitellogenin, field study |
