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(M/EH017) Comparing cadmium accumulation in six populations of Artemia through the application of a two-compartment kinetic model. Sarabia, Raquel1, Del Ramo, Jose1, Varo, Inma2, Amat, Francisco2, Pastor, Agustin3, Díaz-Mayans, Javier1, Torreblanca, Amparo1, 1 2 3 ABSTRACT- The accumulation, and subsequent elimination of cadmium were determined in six populations of Artemia from laboratory experiments. A first-order kinetic model was used to calculate uptake (K1) and elimination (K2) constants from which the bioconcentration factors (BCFs) and depuration half-life (t 1/2) could be predicted.Due to the diversity in cadmium tolerance among populations, different cadmium exposure concentrations (0.1, 0.5, 1 and 10 mg/L) were used in the accumulation phase. Three hundred adult individuals from each population were exposed to cadmium and, at the end of the uptake phase, surviving individuals were rinsed in clean water for the elimination study. A steady-state was reached within 300 hr of cadmium exposure in all populations studied. Close agreement was found between the actual and predicted values suggesting that simple two- compartment (animal-water) models can be employed to predict accurate estimates of toxicokinetic parameters in Artemia. Finally, BCFs and half-lives of cadmium in the six populations of Artemia were compared. Key words: Artemia, kinetic model, cadmium, accumulation |
