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(128) Comparison of the abilities of effluents from two refineries and a municipal wastewater plant to cause estrogenic and anti-estrogenic responses in rainbow trout. Sherry, James1, Munro, Scott2, Moran, Tim3, Kierstead, Ted3, Hansen, Peter4, Hock, Berthold5, 1 2 3 4 5 ABSTRACT- The egg yolk protein vitellogenin (Vg) has gained acceptance as an indicator of exposure to environmental estrogens. Data from some previous experiments in our laboratory indicated that the final waste water from an Ontario refinery may have the ability to induce Vg in rainbow trout and in cultured trout hepatocytes. Those early results were obtained from a 15-d static renewal bioassay with limited replication (n=5), in which the effluent in the individual aquaria was partially (33.3%) renewed on alternate days. For the present study we collected fresh wastewater daily from two refineries, a municipal wastewater plant, and a reference upriver site. The ability of the wastewater to induce Vg was assessed in a 21-d static renewal bioassay (n=15) in which the exposure solutions were completely changed on a daily basis. Fish (n=30) were caged simultaneously in the recipient water close to the point of discharge from each facility, and also at the reference site. Half of the caged fish were primed with 17[:bgr:-estradiol to also test for depression of Vg production at the various wastewater impacted sites compared to the reference site. Key words: Estrogen, Vitellogenin, Refinery, Effluent |
