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(M/EH083) Predicting uptake of organic contaminants in terrestrial organisms using SPME in a soil system. van der Wal, Leon1,2, Hol, Suzanne1, van Gestel, Kees2, Sinnige, Theo1, Hermens, Joop1, 1 2 ABSTRACT- Soil pollution is a wide spread problem which is causing toxicity in terrestrial organisms. Many of these organic contaminants act by means of narcosis, i.e. partitioning of organic compounds into the cell membrane causing disfunctioning of the membrane and therefore toxicity. In order to predict the internal concentrations and bioavailability of organic contaminants in terrestrial organisms several extraction techniques including solvent mixtures and solid phases such as a C18-coated membrane disk have been applied. Our work focusses on the potential applicability of Solid Phase Micro Extraction (SPME) to estimate internal concentrations. A artificial soil (OECD) has been spiked with individual chlorobenzenes and a mixture of these compounds. Enchytraeids have been exposed to this contaminated OECD-soil and concentrations of the contaminants were measured until equilibrium was reached within the organism. Enchytraeids were also exposed to a natural soil (Volgermeerpolder) containing a mixture of contaminants (chlorobenzenes, PCBs and PAHs) with a wide range of log Kow. Concentrations were measured until equilibrium was reached for most contaminants present in this contaminated natural soil. SPME-fibers were exposed to both the spiked OECD-soil and Volgermeerpolder-soil and measured until concentrations in the fibers reached equilibrium. Results of this research will be presented. Key words: spme, soil, biomimetic-extraction, enchytraeids |
