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(201) Biomonitoring of gamma ray toxicity using recombinant fluorescent mammalian cells. Gu, Man Bock1, Kim, Eun Jin1, Lee, Young1, 1 ABSTRACT- The toxic effect of gamma rays was monitored using a recombinant fluorescent chinese hamster ovary (CHO) cell line, KFC-A10. It contains the recombinant plasmid(pKCFG), which was the c-fos SRE promoter region fused with the green fluorescence protein (GFP), constructed in this study for detecting toxicity of gamma ray intensity. It is known that c-Fos is involved in proliferation and differentiation of signal transductions and overexpression of this gene can lead cell to death under the toxic conditions. Therefore, pKCFG is induced by toxic chemicals, especially DNA damage agents, and produces GFP under these toxic conditions. Through this study, it was shown that the KFC-A10 cell line had a dose dependent GFP expression pattern due to various intensities of gamma rays. Therefore this study proved the possibility to monitor the hazardous effects of gamma rays using c-fos SRE with GFP as a reporter in mammalian cells and has also shown that mammalian cells have higher sensitivity compared with prokaryotic biomonitoring system. <Acknowledgement> This work was supported by the Science and Technology Policy Institute (STEPI) at the Kwangju Institute of Science and Technology (K-JIST). Authors are grateful for their support. Key words: gamma ray toxicity, c-Fos, GFP, mammalian cell |
