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(M/EH021) Integration of Alternative Ecotoxicological Models for the Evaluation of Pentachlorophenol. Repetto, Guillermo1, Jos, Angeles1, Hazen, María2, Molero, María2, del Peso, Ana1, Salguero, Manuel1, del Castillo, Pedro2, Rodríguez-Vicente, María1, Repetto, Manuel1, 1 2 ABSTRACT- Bioassays are used in Ecotoxicology and Environmental Toxicology to provide information for risk assessment evaluation of new chemicals and to investigate their effects and mechanisms of action; in addition, ecotoxicological models are used for the detection, control and monitoring of the presence of pollutants in the environment. As a single bioassay will never provide a full picture of the quality of the environment, a representative, cost-effective and quantitative test battery should be derive. The effects of the insecticide Pentachlorophenol were studied using a battery of ecotoxicological model systems, including inhibition of bioluminiscence in the marine bacterium Vibrio fischeri, growth inhibition of the alga Chlorella vulgaris, micronuclei induction in the plant Allium cepa, the inhibition of MTT reduction in Vero cells, the inhibition of cell growth in the salmonid fish cell line RTG-2, derived from the gonad of rainbow trout (Oncorhynchus mykiss), and the immobilization of the cladoceran Daphnia magna. This uncoupler of oxidative phosphorilation was very toxic for all the model systems. The most sensitive system was the induction of micronuclei in Allium cepa, followed by bioluminescence inhibition on Vibrio fischeri bacteria at 60 min. Daphnia magna and Chlorella vulgaris showed intermedian sensitivity. The less sensitive systems were MTT reduction on Vero monkey cells at 24 hr and inhibition of cell proliferation on the RTG-2 cells. Key words: Alternatives, pentachlorophenol, ecotoxicology, battery |
