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(T/EH060) Heavy metal influences on basic mechanisms of active transport of Na+, K+ in neurones. Dvoretsky, Anatoly1, Yegorova, Elena1, Severinovskaya, Elena1, Zaychenko, Elena1, 1 ABSTRACT- In regions with developed industry an environment pollution with high-toxic compounds of heavy metals is observed. Entering the surroundings heavy metals migrate and are distributed in a biota accordingly to specific regularities. Being accumulated in water and food raw stuff they enter into a human organism, causing several diseases, which include neurological ones with their specific significance and role for a human life. Taking it into account influence of widespread high-toxic metals such as lead, cuprum and cobalt on basic mechanisms of active ion transport of Na+ and K+ in neurones of rat brain was under investigation. For this toxic metal salts were given to animals for a month with drinking water in concentrations, correspondent to real levels of industrial and agricultural contaminations. It has been shown that heavy metal salts used in experiments differently influence on transmembrane ion transport in nervous cells. Thus, loading of experimental animal organisms with salts of lead and cuprum inhibites the active transport of Na+ and K+ in brain membranes. At the same time cobalt salts don't cause essential disturbances of this process. To study molecular mechanisms of heavy metals effect on transmembrane ion transport some parameters reflecting the neuronal membrane state were under investigation. Data obtained in these experiments show an activation of lipid peroxidation processes. Perhaps, one of leading mechanisms of toxicological disturbance of active transport of Na+ and K+ is change of physical-chemical properties of neuronal membrane apparatus, resulting in disenergization of a cell and in Na+, K+-pump inhibition. Key words: heavy metals, neuronal membranes, active transport, lipid peroxidation |
