|
(M/EH022) Toxicity of the S-triazine herbicide Gesatop® to zebrafish larvae (Danio rerio). Arellano, Juana1, Sarasquete, Carmen2, Gavaia, Paulo3, Moreno, Manuela1, Arufe, Isabel1, 1 2 3 ABSTRACT- Simazine, the active ingredient of the commercial herbicide Gesatop®, is a pre-emergence herbicide used for control of broad-leaved and grassy weeds on a variety of deep-rooted crops such as artichokes, asparagus, berry crops, broad beans, citrus, pome and stone fruits orchards, and others. Simazine is also used as a non-selective herbicide for weed control in industrial areas. The objective of this study was to test the effects of this herbicide on survival and growth of zebrafish larvae (Danio rerio). Ten larvae per replicate were exposed in a static renewal system for seven days to different concentrations (range 0.625-100 Key words: Herbicide, simazine, zebrafish |
g/L) of the commercial formulation Gesatop® 90GW at a temperature of 26±1 °C, pH 7.7 and a photoperiod of 12 h. Three replicates per each concentration and the water control were used. Test results were analysed using a hypothesis testing approach (Dunnett′s test). Survival was not significantly reduced for the zebrafish exposed to concentrations tested and did not affect larval growth. Control survival was >85%.