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(M/EH085) Extraction of pollutants from soil with regard to bioavailability and general toxicity. Bergknut, Magnus1, Ragnvaldsson, Daniel2, Leffler, Per2, Tysklind, Mats1, 1 2 ABSTRACT- This is the first part of an ongoing study concerning the analysis and evaluation of complex mixtures of contaminants in environmental samples. The final goal will be the possibility to correlate toxicity with chemical characterisation data. The first step in the approach is to evaluate the extraction of pollutants from the sample. An extraction technique that is relevant to the true concentrations in the sample as well as the bioavailability of the pollutants and where the extract is compatible with bioassay techniques is desired. In this study water leeching and Pressurised Liquid Extraction with different solvents ranging from Hexane/Acetone to Water was investigated. The extracts where evaluated with GC/MS and LC/UV. The toxicity of the extracts where screened by neutral red assay using the cell line L-929, a mouse fibrocyte from lung tissue, as a measurement of general toxicity. Preliminary results show that water leaching is not sufficient to induce toxicity in all samples while Pressurised Liquid Extraction with water clearly showed toxic activity. Pressurised Liquid Extraction with organic solvent proved to be the most effective but with the drawback of not being able to expose all of the extracted compounds to the cells due to solubility problems. These preliminary results also give an indication of the bioavailable fraction of the sample, as bioavailability is strongly associated with water solubility. Key words: Extraction, bioavailability, toxicity |
