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(121) Fractionation and chemical analysis of watercourse SPMD extracts. Kind, Tobias1, Vrana, Brano1, Brack, Werner1, Schüürmann, Gerrit1, 1 ABSTRACT- Semipermeable membrane devices (SPMDs) are used as a model for the bioconcentration of organic substances. Recent studies with SPMD extracts from areas in Bitterfeld/Germany showed that SPMDs accumulate a large subset of neutral lipophilic organic compounds (PCBs, PAHs, HCHs) as well as more hydrophilic compounds like chloronitrobenzenes, chlorophenoles, sulfur-PAHs, oxy-PAHs, triazines and nonylphenoles. In this study concentrations of selected marker substances from SPMDs, algae, water and sediment were determined and compared using a correlation analysis approach. Compounds from different substance classes were chosen by toxicity data and their classification as regulated substances. In addition also marker substances from the Bitterfeld area which were found in independent studies using Toxicity Identification Evaluation (TIE) techniques were included. The complex mixtures were fractionated with gel permeation chromatography, flash-chromatography and HPLC, and the sub-fractions were analyzed with GC/MS. The discussion focuses on elucidating the suitability of SPMDs to enrich contaminants with different physicochemical properties. To this end, data from different compartments are compared and flux directions with the help of fugacities are calculated. This leads to a better understanding of source and sink processes in the environment and demonstrates a useful tool for hazard assessments. Key words: SPMD, algae, GC/MS, fractionation |
