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(T/MF200) PAH distribution in marine sediments from Guanabara Bay mangrove areas (Brazil) using HPLC Fluorescence. Pivato, Mauro1, Verçosa Carvalherira, Lucia2, Manente, Sabrina1, Bragadin, Marcantonio1, Perin, Guido1, 1 2 ABSTRACT- The presence of PAHs is one of the most reliable index of pollution as they are the main products of incomplete combustions and organic substrates decomposition. With this research we wanted to quantify the PAHs content in the bottom sediments of the Guanabara Bay (Rio de Janeiro - Brazil) collected before the disaster, in which a big oil spilling took place. These sediments, in the future, will be compared with those collected in the same parts of the bay, after the big oil accident, with regard to the endangered mangrove and wetland areas. The sediment and water sampling were done in the frame of a ten years Italy-Brazil research project focused on monitoring and evaluating the different behaviours between a temperate and a tropical environmental system. Contaminants are extracted with n-hexane/dicloromethane mixture in a sonication bath; the extract, afer concentration, has being eluted through a multilayer clean-up separation column, packed with sodium sulphate, activated copper, Florisil, neutral alumina and silica. By eluting two times with n-hexane and once with n-hexane/dicloromethane (3:2), three fractions are obtained which were quantified by HPLC with fluorescence detector. The results obtained show, as expected, the main polluted are the ones interested by the oil-ship and cargoes crossing but, surprisingly, some areas far from those involved by the direct pollution. Key words: PAH, Oil spilling, Water pollution, HPLC/ Fluorescence |
