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(283) Dietary accumulation, bioconcentration, and tissue distribution of perfluorinated surfactants in rainbow trout. Martin, Jonathan1, Solomon, Keith1, Mabury, Scott2, Muir, Derek3, 1 2 3 ABSTRACT- Perfluorinated surfactants (PFSs), including carboxylates and sulfonates, have been used for decades in a wide array of commercial products and industrial applications. Presently, perfluorooctanesulfonate (PFOS) can be detected at ug L-1 concentrations in wildlife and in the general human population; while the environmental distribution of other PFSs remains unreported. As a result, regulatory agencies are implementing strategies to control PFS use, and the primary manufacturer has voluntarily agreed to phase out the manufacture of PFOS and some related materials. However, the intrinsic stability of PFSs suggests they will persist in the environment and the route of exposure and environmental fate of PFSs is largely unknown. In an effort to understand PFS accumulation in the aquatic environment, we used juvenile rainbow trout (Oncorhynchus mykiss) to conduct dietary accumulation and bioconcentration tests with perfluorinated sulfonates (4,6, and 8 carbons) and carboxylates (5-14 carbons). For both accumulation tests, liver and whole body concentrations were monitored over an uptake and depuration phase. Tissues were homogenized in alkaline water and PFSs were extracted into methyl-tert-butyl ether as ion pairs with tetra-butyl ammonium hydrogen sulfate. Analysis was done using HPLC/MS/MS on a Quattro LC acquiring in multiple reaction monitoring mode. In general, BAFs and BCFs increased with perfluorinated chain length, however, the initial rate of uptake was delayed for the longest perfluorocarboxylate. Whole body half-lives were much less than what has been reported for humans, likely a result of elimination through the gills. Tissue distribution studies with 100 g rainbow trout showed that PFSs accumulate preferentially in blood plasma, liver, and bile, suggesting enterohepatic recirculation as the mode of bioaccumulation. Key words: perfluorinated, bioaccumulation, lc/ms/ms, rainbow trout |
