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(T/EH076) Development of an analytical method to analyze steroids in mussels. Budzinski, Helene1, Togola, Anne1, Devier, Marie-Helene1, Augagneur, Sylvie1, Narbonne, Jean-François1, Garrigues, Philippe1, 1 ABSTRACT- The scientific community has demonstrated since several years that various chemical contaminants could influence human and animal endocrine systems. The list of these compounds still increases. We find the principal chemical contaminants (PAHs, PCBs, pesticides, Phtalates, Metals, Alkylphenols, Dioxines, ect.), synthetic steroids and natural oestrogens (present in soja and dans le soja ou les betterave). If we consider the important diversity of compounds that can be potentially classified as xenoestrogenic compounds, it appears important to study the hormonal metabolism of biological organisms and the endocrine disruption phenomena. The work presented here deals with the development of a methodology to study and quantify steroids in biological organisms. In a first step we have focussed the development on mussels, as they are classical sentinel organisms studied frequently in environmental monitoring programs. Various steps of extraction (SPE extraction) and purification (SPE purification) have been developed. The final identification and quantification is performed either by GC/MS or LC/MS. This methodology enables the study of the testosterone metabolism in aquatic biological organisms. These developments have been applied to the study of hormonal metabolism in mussels in the Arcachon Bay (Atlantic South -West coast of France). Key words: estrogenic effects, testosterone, mussels, steroids |
