|
(M/FF220) Determination of environmental compounds producing goiter and thyroidal effects. Cerrillo, I.1, Cáceres, M.E.2, Rivas, A.1, Granada, A.1, Rosell, J.2, Olea-Serrano, F.1, Escobar, F.3, 1 2 3 ABSTRACT- A large number of agents in the environment are known to interfere with thyroid gland morphology and function. A relationship has been hypothesized between adverse effects on human and wildlife health and a number of chemical substances (Endocrine disrupters, ED) capable of altering hormonal homeostasis. EDs may enter via food, water or air and bioaccumulate in fat. The purpose of the present study is to develop a methodology for the quantification of EDs from cervical fat tissue of patients with diverse thyroid diseases. A 0.2 g aliquot of fat was dissolved in hexane and eluted in a glass column filled with alumina. The eluted obtained was concentrated to a volume of 500 uL and then injected into a semipreparative HPLC. Sixty fat samples were analyzed for organochlorine pesticides and brominated compounds using gas chromatography with an electron capture detector (GC-ECD). Eighty three percent of the samples were from women. All the samples studied were positive for one or more chemical. The most predominant chlorinated pesticides were DDE and HCB, present in 96 and 92 % of the tested samples respectively. The frequency of appearance in decreasing order was: p,p'DDE, HCB, endosulfan-ether, chlordane, aldrin, endosulfan-diol, lindane, endosulfan-lactone, o,p'DDT, p,p'DDT, endosulfan-II, endosulfan-I, endrin, dieldrin and endosulfan sulfate. Among polybrominated diphenyl ethers (PBDEs), bis-4 bromine phenyl ether, 4 bromine diphenyl ether and penta bromine phenyl ether were the most frequently found. The most sensitive end points of PBDE toxicity in vivo are effects on thyroid function in rats and mice. Therefore the presumption is that these EDs may disrupt normal endocrine function in humans, which can lead to thyroid hyperplasia and alteration of thyroid hormone production. Key words: Thyroid, organochlorine pesticides, polybrominated biphenyl ethers |
