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(W/MF207) Organochlorine pesticides in human tissues and blood. Olea-Serrano, F.1, Botella, B.1, Cerrillo, I.1, Torres, B.1, Fontes, J.2, Torné, P.1, Lorenzo, M.3, Pedraza, V.1, 1 2 3 ABSTRACT- Organochlorine pesticides, polychlorinated biphenyls (PCBs) are widely distributed halogenate aromatic compounds which persistently contaminate the environment. Lipophilic xenobiotics are habitually stored in the fatty tissue. An analysis was performed on samples of fat tissue and serum obtained from one hundred women with different diseases who were undergoing surgical treatments; the mean age was 53 years. Adipose tissue and serum collected during surgical treatment at Granada University Hospital and Almeria Hospital was placed into a glass vial on ice, coded, and frozen to -70°C, always within 30 min of being excised, and stored at the same temperature at the Laboratory of Medical Investigations until they were processed. A liquid-liquid extraction for serum and liquid-solid extraction for fat tissue were performed. The cleaning of the sample before gas chromatography/mass spectrometry (GC/MS) used HPLC technique. Among other pesticides, aldrin, dieldrin, DDT and its metabolites, lindane, methoxychlor and endosulfan were identified. The presence of these products was confirmed by mass spectrometry. The identification and quantification of these organochlorine molecules is important because they have estrogenic effects. Most of the samples contained p,p'DDD, o,p'DDT and p,p'DDT, while p,p'DDE was detected in 100%. Other pesticides and their derivatives were also widely found. There is a fatty tissue/serum relationship in favour of the former with distribution coefficients of between 15 and 123 for dieldrin and p-p'DDE, respectively, with an inversion of this relationship for endosulfan ether with a value of 0.80. Fatty tissue clearly acts as a reservoir of liposoluble compounds. Key words: organochlorine pesticides, human serum, human fat |
