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(418) Bioaccumulation of lindane and hexachlorobenzene by oligochaetes from artificial and natural standard soils. Bruns, Eric1, Egeler, Philipp1, Römbke, Jörg1, Scheffczyk, Adam1, Spörlein, Peter1, 1 ABSTRACT- The results of tests using a newly standardised test with two model chemicals (lindane, hexachlorobenzene) and four different oligochaete species are presented. Enchytraeids and lumbricids were selected as test organisms due to their important ecological role in the soil compartment and their easy handling. Artificial soil according to the OECD Guideline No. 207 and the natural standard soil LUFA 2.2 were used as test substrates. Test concentrations were based on results of enchytraeid acute and reproduction tests performed with the same species. Accumulation as well as the elimination of the test substances were examined under controlled conditions in a closed test system. The results show that differences in the bioaccumulation of the two model chemicals were observed not only in the two different soil types, but the two different oligochaete families (Enchytraeidae , Lumbricidae) as well differ markedly. For example, all bioaccumulation factors (BAF) determined for enchytraeids were higher compared to the corresponding earthworm results. For lindane, extreme differences in the elimination behaviour were observed between the two earthworm species Lumbricus rubellus and Eisenia fetida. In addition, no correlation between terrestrial and aquatic data could be found. In the whole project, sixteen bioaccumulation tests, each one consisting of an uptake and an elimination phase, were performed. The results showed clearly, that the developed test method is useful and delivers reproducible results. A draft guideline for this standardised and validated test system for bioaccumulation tests with terrestrial oligochaete worms will be presented in the near future. Key words: bioaccumulation, soil, oligochaetes, test guideline |
