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(T/EH081) Toxicity assay of carbofuran and its degraded compound, phenolcarbofuran using FETAX and Microtox. Hwang, In Young1, Park, Jeong-Gue2, Lee, Soon Ae1, Lee, Sung Kyu3, 1 2 3 ABSTRACT- Carbofuran is known as a pesticide shown toxic effects on aquatic and terrestrial aniamals. As one of efforts to assess the ecological risk of carbofuran, we assayed the environmental toxicity of carbofuran and its major degraded compound, phenolcarbofuran (2,3- Dihydro-2,2-dimethyl-7-benzofuranol) using both FETAX (frog embryo teratogenesis assay-Xenopus) and Microtox methods. The LC50 of carbofuran and phenolcarbofuran were 43.4ppm and 160.1ppm, respectively. And their teratogenic EC50 for whole body of embryos were 0.11ppm and 7.58ppm, respectively. In the 4-day cultured embryos, it had been found severe ventral tail flexure, edema and abnormal pigment in embryos exposed to carbofuran, but much less deformities in embryos exposed to phenolcarbofuran. These findings indicate that a parent compound, carbofuran is stronger teratogen than its degraded compound, phenolcarbofuran. In addition, carbofuran's Microtox EC50 was 12.1ppm at 30min reading and it is similar to other common inseticide's toxic level. That means carbofuran may be more sensitive to frog embryos than luminescent microorganism and other aquatic species. Key words: carbofuran, phenolcarbofuran, FETAX, Microtox |
