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(T/EH085) A pilot plant for in situ monitoring of mutagenic by-products produced by disinfection of surface water . Monarca, S.1, Zani, C.1, Zerbini, I.1, Guzzella, L.2, 1 2 ABSTRACT- A battery of in vivo short-term genotoxicity tests revealing different genetic end-points was set for application in the study of surface drinking water treated with different disinfectants. Field studies were performed at a pilot drinking water treatment plant using lake water after sedimentation and filtration. This water supplied four stainless steel basins: three basins were disinfected respectively with hypochlorite, chlorine dioxide and acid peracetic and a fourth basin with untreated lake water was used as control. In these basins fishes (Carpio cyprinus), mussels (Dreissena polymorpha) and plants were exposed in situ. In particular, the in vivo tests performed directly by exposing animal (fish and mussels) and plant (Tradescantia, Allium cepa and Vicia faba) bioindicators: 1. micronuclei test in Tradescantia pollen (Tradescantia/micronuclei test using clone # 4430, hybrid of T. hirsutiflora and T. subacaulis); 2. chromosomal aberrations in root cells of Allium cepa (Allium cepa test) and toxicity evaluation by means of root length; 3. micronuclei test in root cells of Vicia faba; 4. micronuclei test and DNA damage test (Comet test) in erythrocytes of fish or haemocytes of mussels; 5. assays for enzymatic modifications in fish and mollusc tissues. Preliminary results showed promising applicability of plant and fish tests for monitoring disinfection by-products in surface water. Key words: Peracetic acid, Disinfection by-products, Mutagenicity |
