PARENT SESSION 22 - Biochemical, Cellular and Molecular Background of Biomarkers 8:00 AM to 6:30 PM, Monday, 13 May 2002 Exhibition Area22-01 - Study on the Oxidative Stress Induced by UV and PAHs Using DNA integrity as a Biomarker. Zhang, Xiaowei*,1, Wu, Rudolf S S1, Lam, Paul K S1, 1 Department of Biology and Chemistry. City University of Hong Kong, Hong Kong, none
FULL ABSTRACT 22-02 - Application of cellular biomarkers in a monitoring programme along the Western Mediterranean sea. Zorita, Izaskun1, Apraiz, Itxaso1, Barrouillet, Marie1, Bilbao, Eider1, Cancio, Ibon1, Soto, Manu1, Orbea, Amaia1, Cajaraville, Miren*,1, 1 Dept. Zoology and Animal Cell Dynamics. Univ. Basque Country., Bilbo., Basque Country (Spain)
FULL ABSTRACT 22-03 - Seasonal biomarker variations relative to physiological indicators and chemical contaminant levels in Mediterranean mussels (Mytilus galloprovincialis). Bodin, N.*,1, Stanisière, J.2, Miralles, F.2, Chavanon, F.2, Ravel, C.2, Menard, D.1, Budzinski, H.3, Pfohl Leskowicz, A.4, Amat, A.4, Cherel, Y.5, Minier, C.6, Bocquené, G.1, Burgeot, T.1, 1 Ifremer, Nantes, France2 Ifremer, La Seine sur Mer, France3 LPTC University of Bordeaux, Talence, France4 ENSAT, Auzeville-Tolosane, France5 ENV, Nantes, France6 University of Le Havre, Le Havre, France
FULL ABSTRACT 22-04 - Use of histopathologic indicators in evaluating environmental impact on chubs and brown trouts. Paolini, Alessandra*,1, Schwaiger, Julia2, D'Angelo, Anna1, Giansante, Carla1, Berti, Miriam1, Ferri, Nicola1, 1 Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G.Caporale", Teramo, Italy2 Bavarian State Agency for Water Management, Wielenbach, Germany
FULL ABSTRACT 22-05 - Biomarker studies of perch (Perca fluviatilis) in a chronically polluted gradient in Stockholm's waterways. Linderoth, Maria*,1, Sundberg, Henrik1, Noaksson, Erik1, Hansson, Tomas1, Tjärnlund, Ulla1, Liewenborg, Birgitta1, kerman, Gun1, Balk, Lennart1, 1 Laboratory for Aquatic Ecotoxicology, Institute of Applied Environmental Research Stockholm University, Stockholm, Sweden
FULL ABSTRACT 22-06 - Biomarkers for sublethal stress in green lipped mussel (Perna viridis) exposed to various dose regimes of Polycyclic Aromatic Hydrocarbons (PAHs) and Organo Chlorine (OC) pesticides. Mak, E.*,1, Richardson, B.J.1, Lam, P.K.S.1, 1 City University of Hong Kong, /, /
FULL ABSTRACT 22-07 - Effects of diazinon on AChE activity of the terrestrial isopod Porcellio scaber (Isopoda, Crustacea). Trebse, Polonca*,1, Drobne, Damjana2, 1 Nova Gorica Polytechnic, Laboratory for Environmental Reeserch, Vipavska 13, Nova Gorica, Slovenia2 Department of Biology, University of Ljubljana, Vecna pot 111, Ljubljana, Slovenia
FULL ABSTRACT 22-08 - Increased protein oxidation in fish from a contaminated site. Carney Almroth, Bethanie*,1, Sturve, Joachim1, Förlin, Lars1, 1 Göteborg University, Göteborg, Sweden
FULL ABSTRACT 22-09 - Molecular Responses to Chemical Pollutants in Sparus Auratus and Scapharca Inaequivalvis of Adriatic Sea. Isani, G.*,1, Monari, M.1, Andreani, G.1, Serrazanetti, G.P.1, Cattani, O.1, Trinchero, A.1, Carpenè, E.1, 1 University of Bologna, Bologna, /, Italy
FULL ABSTRACT 22-10 - Reduction of EROD activity with age in Atlantic tomcods from the St. Lawrence Estuary, Canada. Couillard, Catherine*,1, Légaré, Benoît1, Lebeuf, Michel1, Poirier, Guy2, 1 Department of Fisheries and Oceans, Maurice Lamontagne Institute, Mont-Joli, Quebec2 CHUL Research Center, Québec, Quebec
FULL ABSTRACT 22-11 - Characterization of exposure and effect biomarkers in the European eel Anguilla anguilla. Corsi, Ilaria*,1, Mariottini, Michela1, Bonacci, Stefano1, Focardi, Silvano1, Frenzilli, Giada2, Nigro, Marco2, Gorbi, Stefania3, Regoli, Francesco3, 1 Dipartimento di Scienze Ambientali, Siena, Italy2 Dipartimento Morfologia Umana e Biologia Applicata, Pisa, Italy3 Istituto Biologia e Genetica, Ancona, Italy
FULL ABSTRACT 22-12 - Optimization of techniques to measure peroxisome proliferation in mussels. Orbea, Amaia*,1, Cajaraville, Miren1, 1 University of the Basque Country, Bilbao, Spain, Spain
FULL ABSTRACT 22-13 - Immunotoxicological response of terrestrial and aquatic invertebrates following in vitro exposure to trace elements. Sauvé, Sébastien*,1, Fournier, Michel2, 1 Université de Montréal, Montreal, QC, Canada2 INRS-Institut Armand-Frappier-Santé humaine, Montreal, QC, Canada
FULL ABSTRACT 22-14 - Biomarker responses and chemical analysis in fish indicate marked release of PAH from car tyre rubber. Förlin, Lars*,1, Stephensen, Eiríkur1, Adolfsson-Erici, Margaretha2, Parkkonen, Jari1, Sturve, Joachim1, 1 Department of Zoology, Zoophysiology, Goteborg, Sweden2 Institute of Applied Environmental Research, Stockholm, Sweden
FULL ABSTRACT 22-15 - Antioxidant responses and lipid peroxidation in fish upon exposure to Chattonella spp.. Woo, P.S.*,1, Liu, W.H.1, Wu, S.S.1, 1 City University of Hong Kong. 83 Tat Chee Avenue, Kowloon, Hong Kong, /
FULL ABSTRACT 22-16 - CITYFISH: Effects on chub (Leuciscus cephalus) of chronic exposure to sub-lethal levels of complex pollutant mixtures in an urban river in Italy. McKenzie, D.2, Agradi, E.3, Rocco, R.1, Stopelli, B.1, Regondi, S.3, Taylor, E.2, Ceradini, S.*,1, 2 School of Biosciences, Birmingham, United Kingdom.3 Institute of Pharmacological Sciences, Milano, Italy.1 CESI, Segrate (MI), Italy.
FULL ABSTRACT 22-17 - Chemical profile and contribution of PAHs to in vitro toxic potencies of urban particulate matter samples. Machala, Miroslav*,1, Neca, Jiri1, Ciganek, Miroslav1, Blaha, Ludek1,2, Janosek, Jaroslav2, Kapplova, Petra1,2, Binkova, Blanka3, Vondracek, Jan1,4, 1 Veterinary Research Institute, Brno, Czech Republic2 RECETOX-TOCOEN & Associates, Brno, Czech Republic3 Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, CAS, Prague, Czech Republic4 Institute of Biophysycs, CAS, Brno, Czech Republic
FULL ABSTRACT 22-18 - A new approch using a biomarker pattern in plants exposed to herbicides. Ravn, H.*,1, Løkke, H.1, 1 National Environmental Research Institute, Silkeborg, Denmark
FULL ABSTRACT 22-19 - Time course of expression of metallothionein-like proteins in Chironomus riparius larvae exposed to a sub-lethal concentration of cadmium. Prenner, Monika*,1, Leung, Kenneth2, Crane, Mark1, 1 School of Biological Sciences, Royal Holloway, University of London, Egham, UK2 Department of Ecology and Biodiversity, The University of Hong Kong, Hong Kong
FULL ABSTRACT 22-20 - CityFish: Correlation between PAH exposure and biomarkers in caged carp and feral bream . Verweij, Frank*,1, Ceradini, Sergio2, Agradi, Elisabetta3, Van der Oost, Ron1, 1 OMEGAM Environmental Research Institute, P.O. 94685, Amsterdam, The Netherlands2 CESI, Via R. Rubattino 54, Milan, Italy3 The University of Milan, Via Balzaretti 9, Milan, Italy
FULL ABSTRACT 22-21 - Fluorimetric measurement of EROD activity. Caution about the spectral characteristics of the standards used. Radenac, Gilles1, Coteur, Geoffroy2, Danis, Bruno2, Dubois, Philippe2, Warnau, Michel*,2,3, 1 Lab. de Biologie & Environnement Marins- EA 3168 - Univ. La Rochelle - Institut de la Mer - Quai Lazaret - Port des Minimes, La Rochelle, France2 Laboratoire de Biologie Marine, Université Libre de Bruxelles - Av. F.D. Roosevelt, Brussels, Belgium3 IAEA - Marine Environment Laboratory, 4 Quai Antoine Ier, BP 800, Monaco, Principality of Monaco
FULL ABSTRACT 22-22 - Internal multielement analysis as a biomaker of metal exposure. Scott-Fordsmand, Janeck*,1, Odegard, Knut2, 1 National Environmental Research Institute, Silkeborg, Denmark2 Aquateam, Oslo, Norway
FULL ABSTRACT 22-23 - An inducible intron-containing hsp70-gene in the diplopod Tachypodoiulus niger? Knigge, Thomas*,1, Bachmann, Lutz2, Köhler, Heinz-R.1, 1 Animal Physiological Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, Tübingen, Germany2 Zoological Museum, University of Oslo, Sars gate 1, Oslo, Norway
FULL ABSTRACT 22-24 - An approach to metallothioneins in isopods. Knigge, Thomas*,1, Sterenborg, Ingrid2, van Straalen, Nico2, Köhler, Heinz-R.1, 1 Animal Physiological Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, Tübingen, Germany2 Department of Ecology and Ecotoxicology, Vrije Universiteit, De Boelelaan 1087, Amsterdam, The Netherlands
FULL ABSTRACT 22-25 - Evaluation of different subcellular and cellular test systems for an ecotoxicological risk analysis. Stock, Frauke*,1, Jastorff, Bernd1, 1 UFT, Center for Environmental Research and Technology, Leobener Str., Bremen, Germany
FULL ABSTRACT 22-26 - Sterols content in agricultural soils and their potential use as biochemical indicators. Puglisi, Edoardo*,1, Nicelli, Marco1, Capri, Ettore1, Trevisan, Marco1, Del Re, Attilio1, 1 Istituto di Chimica Agraria ed Ambientale, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, Italy
FULL ABSTRACT 22-27 - Effects of potentially pollutant agents on fatty acid methyl esters profiles in agricultural soils. Puglisi, Edoardo*,1, Nicelli, Marco1, Capri, Ettore1, Trevisan, Marco1, Del Re, Attilio1, 1 Istituto di Chimica Agraria ed Ambientale, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, Italy
FULL ABSTRACT 22-28 - CITY FISH - The biochemical responses (Biomarker) in fish from waterways in urban area's. Mierke, Wilma1, Verweij, Frank2, Ceradini, Sergio3, Agradi, Elisabetta4, Fischer, Birgit1, Hansen, Peter-1, Unruh, Eckehardt*,1, 1 Technische Universitaet Berlin, Dept. of Ecotoxicology, Berlin, Germany2 OMEGAM, Environmental Toxicology, Amsterdam, Netherlands3 CESI SpA, Segrate (MI), Italy4 Universita di Milano, Dipartimento di Scienze Farmacologiche, Milano, Italy
FULL ABSTRACT 22-29 - Methodological and biological aspects to be considered in acetylcholinesterase reactivation assays using 2-PAM. Monserrat, José*,1, Bianchini, Adalto1, 1 Fundação Universidade Federal do Rio Grande, Rio Grande, RS, BRAZIL
FULL ABSTRACT 22-30 - CITYFISH: Occurrence of DNA strand breaks in liver of chub from UK rivers. Winter, Matt*,1, Hayes, Ruth1, Ceradini, Sergio2, Butler, Pat1, Taylor, Ted1, Chipman, James1, 1 School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK2 CESI Environmental Unit, Milan, Italy
FULL ABSTRACT 22-31 - HSP70 in rainbow trout primary cultures in response to 2,4-DCA exposure: an in vitro/in vivo correlation. Kilemade, M.*,1, Mothersill, C.2, 1 University College Cork, Cork, Ireland2 Dublin Institute of Technology, Dublin, Ireland
FULL ABSTRACT 22-32 - Detection of DNA strand breaks in fish cells exposed in vitro and in vivo to cadmium. Kilemade, M.*,1, O'Brien, N.2, O’Halloran, J.1, van Pelt, F.3, Sheehan, D.4, Mothersill, C.5, 1 University College Cork, Cork, Ireland2 University College Cork, Cork, Ireland3 University College Cork, Cork, Ireland4 University College Cork, Cork, Ireland5 Dublin Institute of Technology, Dublin, Ireland
FULL ABSTRACT 22-33 - Cellular responses elicited by cyanobacterial toxins in a rainbow trout liver cell line. Teneva, Ivanka*,1, Schirmer, Kristin2, Mladenov, Rumen1, Schüürmann, Gerrit2, 1 University of Plovdiv, Plovdiv, Bulgaria2 UFZ Centre for Environmental Research, Leipzig, Germany
FULL ABSTRACT 22-34 - Determination of metallothionein and related protein levels in fish tissues for monitoring purposes. Van Campenhout, Karen*,1, Goenaga Infante, Heidi2, Adams, Freddy2, Blust, Ronny1, 1 University of Antwerp (RUCA), Groenenborgerlaan 171, Antwerp, Belgium2 Micro and Trace Analysis Center, University of Antwerp (UIA), Universiteitsplein 1, Antwerp, Belgium
FULL ABSTRACT 22-35 - Metallothioneins in the mussel Mytilus galloprovincialis and their use as biomarker for heavy metal pollution. Menge, S.1, Miersch, J.1, Krauss, G.-J.1, Chafik, A.2, Benhra, A.2, Elamrani, M.K.3, Elghazi, I.*,1,3, 1 Martin Luther University, Dep. Biotechnology/Biochemistry, Halle, Germany2 INRH, Ecotoxicology laboratory, Casablanca, Morocco3 Hassan II University, Casablanca, Morocco.
FULL ABSTRACT 22-36 - Stress protein expression and immunological responses in juvenile Chinook salmon (Oncorhynchus tshawytscha) exposed to pesticides. Eder, Kai1, Leutenegger, Christian1, Koehler, Heinz2, Werner, Inge*,1, 1 University of California, Davis, California, USA2 University of Tuebingen, Germany
FULL ABSTRACT 22-37 - Molecular Stress Response, Mortality, and Recovery of the Amphipod Gammarus fossarum (Koch, 1835) Following Single Pulse Exposure to Cadmium. Schill, Ralph*,1, Görlitz, Holger1, Köhler, Heinz-R.1, 1 University of Tübingen, Zoological Institute, Animal Physiological Ecology, Konrad-Adenauer-Str. 20, Tübingen, Germany
FULL ABSTRACT 22-38 - Temporal expression of HSP-70, Acetylcholinesterase and cytochrome P450 in Mytilus galloprovincialis transplanted in a reference and a polluted site. Corsi, Ilaria*,1, Chiea, Riccardo1, Iacocca, Annalisa1, Viarengo, Aldo2, Focardi, Silvano1, 1 University of Siena, Siena, Italy2 University of Piemonte Orientale, Alessandria, Italy
FULL ABSTRACT 22-39 - Temporal expression of HSP-70 in Mytilus galloprovincialis transplanted in a reference and a polluted site. Chiea, Riccardo*,1, Corsi, Ilaria1, Lucidi, Cristina1, Viarengo, Aldo2, Focardi, Silvano1, 1 University of Siena, Siena, Italy2 University of Piemonte Orientale, Alessandria, Italy
FULL ABSTRACT 22-40 - Induction and recovery of intestinal biochemical and quantitative cytological changes in fish by foodborne PAH. Yuen, Bonny*,1, Pollino, Carmel1, Au, Doris1, 1 Center for Coastal Pollution and Conservation, City University of Hong Kong, Kowloon Tong, Hong Kong
FULL ABSTRACT 22-41 - Evaluation of Freshwater Toxicity: Use of a Protozoon-based Bioassay and Comparison with Common Toxicity Tests. Viarengo, Aldo*,1, Evangelisti, Valentina1, Dondero, Francesco1, Mozzone, Sabrina2, Burlando, Bruno1, 1 D.I.S.T.A. , University of Eastern Piedmont, C.so T. Borsalino 54, Alessandria, Italy2 A.R.P.A. Piemonte, Dept. of Alessandria, Environmental Toxicology Lab, Via Trotti, 17, Alessandria, Italy
FULL ABSTRACT 22-42 - Quantitation by Capillary Electrophoresis of Metallothionein in Liver of Aquatic Animal Species. Eberini, Ivano1, Gianazza, Elisabetta1, Regondi, Simona1, McKenzie, David2, Agradi, Elisabetta*,1, 1 Universita' degli Studi di Milano, Milano, Italia2 University of Birmingham, Birmingham, U.K.
FULL ABSTRACT 22-43 - Inhibition of Seed Protein Catabolism and of Shott Protein Anabolism After Exposure to Heavy Metals. Gianazza, Elisabetta1, Tome', Franca1, Di Fabio, Tiziana1, Regondi, Simona1, Wait, Robin2, Agradi, Elisabetta*,1, 1 Universita' degli Studi di Milano, Milano, Italia2 Imperial College of Science, Technology and Medicine, London, U.K.
FULL ABSTRACT 22-44 - Rapid fluorescence techniques for the assessment of toxicological effects on bacterial populations within wastewater treatment plants. Forster, Scott*,1, Snape, Jason2, Lappin-Scott, Hilary1, Porter, Jonathan1, 1 School of Biological Sciences, Exeter, Devon UK2 Brixham Environmental Laboratory, Devon, UK
FULL ABSTRACT 22-45 - Gene Expression Profiling in Metal and PAH-Exposed Daphnia magna. Diener, Lara*,1, Greenberg, Bruce1, Schulte, Patricia2, 1 University of Waterloo, Waterloo, Ontario2 University of British Columbia, Vancouver, British Columbia
FULL ABSTRACT 22-46 - Assessing the toxicity of surfactants to protozoa, fish and mammalian cells. Dayeh, Vivian*,1, Chow, Stephanie1, Schirmer, Kristin2, Lynn, Denis3, Bols, Niels1, 1 University of Waterloo, Waterloo, ON2 UFZ Centre for Environmental Research, Leipzig-Halle, Germany3 University of Guelph, Guelph, Ontario
FULL ABSTRACT 22-47 - Biochemical and hepatocytological markers of effects for PAHs exposure in fish. Au, Doris*,1, 1 Center for Coastal Pollution and Conservation, City University of Hong Kong, 83 Tat Chee Avenue, Hong
FULL ABSTRACT 22-48 - Exposure to pollutant mixtures of chub (Leuciscus cephalus): effects on mRNA stability and translational activation of connexin-43, connexin-32 and Hsp-70 (CITYFISH). Cillo, F*,1, Brevini, T1, Francisci, C1, Ceradini, S2, Chipman, J3, Taylor, E3, 1 Department of Anatomy of Domestic Animals, Embryology Unit, Milan, Italy2 CESI Environment Unit, Milan, Italy3 School of Biosciences, Birmingham, United Kingdom
FULL ABSTRACT 22-49 - Antibodies against fish CYP1A proteins used for purification and development of a quantitative CYP1A-ELISA. Langvik, Magnus1, Nilsen, Bente2, Goksøyr, Anders*,1,2, 1 Department of Molecular Biology, Bergen, Norway2 Biosense Laboratories AS, Bergen, Norway
FULL ABSTRACT 22-50 - In situ sediment biomarkers in Chironomus riparius. Kheir, Rania*,1, Crane, Mark1, Prenner, Monika1, Maycock, Dawn1, Olsen, Tony2, Callaghan, Amanda3, 1 School of Biological Sciences, Royal Holloway University of London, Egham, UK2 Anglian Water, Huntingdon, Cambidgeshire, UK3 School of Animal and Microbial Sciences, University of Reading, Whiteknights PO Box 228, Berkshire, UK
FULL ABSTRACT 22-51 - The differential sensitivity of three invertebrates to copper assessed using biomarkers of exposure. Lowe, David2, Brown, Mark1, Dissanayake, Awantha1, Jones, Malcolm1, Galloway, Tamara1, Depledge, Michael1, Brown, Rebecca*,1, 2 Plymouth Marine Laboratory, Plymouth, Devon1 Plymouth Environmental Research Centre, Plymouth, Devon
FULL ABSTRACT 22-52 - Cellular aggregation as a biomarker of exposure in marine sponges. Brown, Rebecca*,1, Lowe, David2, Jones, Malcolm1, Galloway, Tamara1, Depledge, Michael1, 1 Plymouth Environmental Research Centre, Plymouth, D2 Plymouth Marine Laboratory, Plymouth, Devon
FULL ABSTRACT 22-53 - Metallothionein immunohistochemical levels in liver and gill target cell types of cadmium-exposed turbot (Scophthalmus maximus). Alvarado, Nelva1, Buxens, Amaya2, Amaral, Andre2, Mazon, Luis2, Cancio, Ibon1, Marigomez, Ionan*,1, Soto, Manu1, 1 Dept. of Zoology and Animal Cell Dynamics. Univ. Basque Country., Bilbo., Basque Country (Spain)2 Dept. of Animal Biology and Genetics. Univ. Basque Country., Bilbo., Basque Country (Spain)
FULL ABSTRACT 22-54 - Model slugs to investigate the role of calcium cells in adaptation to chronic metal-pollution. Zaldibar, Beñat*,1, Iturralde, Josune1, Cancio, Ibon1, Marigomez, Ionan1, 1 Dept. of Zoology and Animal Cell Dynamics. Univ. Basque Country., Bilbo., Basque Country (Spain)
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