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(73-06) Nephrotoxicity of the anti-inflammatory drug diclofenac in rainbow trout (Oncorhynchus mykiss). Triebskorn, Rita*,1, Heyd, Andreas1, Casper, Heidemarie2, Köhler, Heinz-R.2, Ferling, Hermann3, Negele, Rolf-Dieter3, Schwaiger, Julia3, 1 Steinbeis-Transfer Center for Ecotoxicology and Ecophysiology, Rottenburg, Germany2 Animal Physiological Ecology, University of Tübingen, Tübingen, Germany3 Bavarian State Agency for Water Management, Wielenbach, Germany ABSTRACT- Twenty-months-old rainbow trout (Oncorhynchus mykiss) were exposed to 1, 5, 20, 100 and 500 Key words: kidney ultrastructure, rainbow trout, diclofenac, anti-rheumatic drug |
g diclofenac/L for 28 days under flow-through conditions. Additional fish were exposed to water and to the solvent (DMSO) exclusively. After the exposure period, samples of different organs were taken for electron microscope studies. In the kidney, prominent reactions were found which include glomerulonephritis-like symptoms in the renal corpuscle (thickening of the basal lamina, increased branching of pedicels, closure of Bowman space by immigrated cells and / or cellular debris), a distinct hyaline droplet degeneration (accumulation of protein) of epithelial cells of the anterior parts of the proximal tubules, dilation and vesiculation of the endoplasmic reticulum in the anterior and posterior parts of the proximal and distal tubules, swelling of mitochondria, an increased lobulation of the nuclei in the distal tubular epithelial cells, and an increased infiltration of the epithlia with macrophages. A semi-quantitative assessment of the observed reactions revealed significant differences already in fish exposed to 1