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(18-27) Effects of 4-nonylphenol on the embryogenesis of the pond snail Lymnaea stagnalis L. Severin, Gabriele*,1, Lalah, Joseph1,2, Behechti, Akbar1, Schramm, Karl-Werner1, 1 GSF-National Research Center for Environment and Health, Neuherberg, Germany2 Maseno University, Maseno, Kenya ABSTRACT- Effects of endocrine disruptors on gastropods have been demonstrated in various studies. The susceptibility of the embryogenesis of the hermaphroditic pulmonate Lymnaea stagnalis L. has been shown in a screening test procedure. Freshly laid egg masses of Lymnaea stagnalis L. were collected and continuously exposed to a radioactive labeled single tertiary isomer of NP, 4(3′-,6′-dimethyl-3′-heptyl)-phenol at a concentration of 105 Key words: embryogenesis, nonylphenol, lymnaea stagnalis |
g/L in a 18 L aquarium until the snails hatched. The NP-concentrations in the water were checked regularly and kept constant by continuous dosing using a HPLC column. The number of fertilised eggs per capsule was counted and six times per week the embryos were examined for deformations, delay in development and mortality. One aquarium remained untreated and served as control. Room temperature during test ranged from 18 to 21 °C. The results of the study will be presented.