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(41-21) Sub-lethal effects of the flame retardants hexabromocyclododecane (HBCDD) and tetrabromobisphenol A (TBBPA) in juvenile rainbow trout. Ronisz, Dan*,1, Farmen Finne, Eivind1, Karlsson, Helena1, Forlin, Lars1, 1 Department of Zoophysiology, Gothenburg university, Gothenburg, Sweden ABSTRACT- Due to extensive computerisation and increased demands of fire-safety, brominated flame retardants leak out in the environment, including the aquatic one, in an accelerating manner. Despite this, sub-lethal effects of these chemicals are generally poorly investigated. In this study a screening of selected biomarkers in juvenile rainbow trout (Oncorhynchus mykiss) was performed after exposure to the brominated flame retardants hexabromocyclododecane (HBCDD) and tetrabromobisphenol A (TBBPA). HBCDD: Fish were injected intraperitoneally and experiments lasted for 5 and 28 days. The short-term experiment showed a dose-related increase in the activity of catalase. A 40% increase in Liver Somatic Index could be observed after 28 days. It is therefore suggested that HBCDD might act in a peroxisome proliferator (PP)-like mode of action in fish. HBCDD did also seem to have an antagonistic effect on CYP1A activity (EROD). TBBPA: The EROD-activity seemed to be inhibited. The activity of glutathione reductase increased after 4, 14 and 28 days suggesting a possible role of TBBPA in inducing oxidative stress. There is clearly a need for further experiments e.g. investigations of the putative PP-like mode of action of HBCDD*, mechanistic studies of EROD inhibition*, and studies of more long-term, low-dose effects of the flame retardants in fish. * These results will hopefully be presented at the conference. Key words: hexabromocyclododecane, tetrabromobisphenol A, biomarkers, catalase |
