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(80-31) Induction of cytochrome P450 in European eel (Anguilla anguilla) exposed to different contaminants. Bonacci, Stefano*,1, Corsi, Ilaria1, Chiea, Riccardo1, Regoli, Francesco2, Focardi, Silvano1, 1 Department of Environmental Sciences, Siena, Italy2 Institute of Biology and Genetic, Ancona, Italy ABSTRACT- The induction of liver ethoxyresorufin O-deethylase (EROD) and benzo[a]pyrene monooxygenase (BaPMO) activities was investigated in the European eel Anguilla anguilla after treatment with B[a]P, Key words: European eel, EROD, BaPMO, contaminants |
-NF, 2,3,7,8-TCDD and PCBs mixture Aroclor 1254, carrying out a dose-response study. Eels were injected intra-peritoneally at increasing doses (B[a]P, 
g/kg body weight), using corn oil as a carrier and sacrificed after 24 hours. The present study investigated EROD and BaPMO activities to achieve three main goals: estimation of dose-response induction in eel with regard to P450 well-known inducers; comparison between the two enzymatic responses and, finally, comparison between results obtained in the present study and from an in situ study, where wild eels were captured without artificial exposure. Results showed that all four compounds produced a dose-dependent induction of both P450 activities, but increases were not linear. The greatest induction occurred in eels treated with B[a]P at the highest dose (50 mg/kg) with a 517-fold and a 77-fold increase in hepatic microsomal EROD and BaPMO activity, respectively. While EROD activities seemed not to reach a plateau, BaPMO showed highest values at the 10 mg/kg dose of Aroclor 1254. Results from the in situ study, showed wild eels with EROD and BaPMO activities higher than those values obtained from control eels in the laboratory study, suggesting the exposition to P450 inducers directly from the environment. The European eel seemed to be particularly sensitive to known P450 inducers with particular regards to B(a)P and 2,3,7,8-TCDD.