PARENT SESSION
40 - Pharmaceuticals in the Environment
8:00 AM to 6:30 PM, Tuesday, 14 May 2002
Exhibition Area

(40-16) Ecotoxicological assessment of Doxycycline in aged pig manure using MultiSpecies Soil Systems (MS.3).

Fernández, Carlos^*,1, Alonso, Carmen¹, Pro, Javier¹, Heranz, Paloma¹, Carbonell, Gregoria¹, Tarazona, José¹, ¹ Laboratory for Ecotoxicology Department of Environment. INIA,.Crta. de La Coruña km 7, Madrid, Spain

ABSTRACT- In the last years, due to the use of veterinary drugs in animal production, and the use of manure in agriculture, the presence of drugs and/or their metabolites in livestock manure involves an environmental risk of high concern. The use of pig manure as agricultural amendment derives to an exposure of terrestrial organisms to these veterinary drugs and/or their metabolites. This paper shows the study of ecotoxicogical effects on terrestrial living organisms (plants, invertebrates and micro-organisms) by the use of a pig manure, containing a residual amount of Doxycycline. The study was performed using the MultiSpecies Soil Systems, with an enforce of 15 days aged pig manure (120 ml, equivalent to 220 kg N/ha), containing residual amounts of a nominal concentration Doxycycline (75 g/ml or 7500 g/ml). The experimental design was completed by comparison with the ecotoxicological effects on MS.3 containing 120 ml of water (control), 120 ml of blank pig manure (blank control), 120 ml of Eprinomectine solution (0.5 %) and 120 ml of Doxycycline water solution (7.5 mg/ml). As end-points, plant biomass, % seed germination (Triticum aestivum and Vicia sativa), % Eisenia foetida mortality and enzymatic activities on soil micro-organisms were used. Doxycycline mobility in soil column was monitored by HPLC analysis of leachates. This work has been founded by the research project UE ERAV-EVKI 1999-00034P and a research collaboration with LABORATORIOS HIPRA S.A. (SPAIN).

Key words: Pig manure, Doxycycline, MultiSpecies Soil Systems (MS.3).