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(80-59) Identifying potential bioaccumulating substances in effluents using SPME-GCMS. De Maagd, P. Gert-Jan*,1, Staeb, Joan1, 1 RIZA, P.O. Box 17, Lelystad, The Netherlands ABSTRACT- One of the effluent assessment parameters within the Dutch Whole Effluent Assessment procedure is bioaccumulation. Based on the potential occurrence of harmful effects in top predators due to bioaccumulation in the food web, the parameter bioaccumulation is regarded as an essential supplement to parameters like acute and chronic toxicity. The parameter bioaccumulation was assessed with Solid Phase Micro Extraction (SPME)-method, which is fast and convenient and can be regarded as a so-called biomimetic extraction. This is an extraction in which only the bioavailable fractions of toxicants are extracted and in which hydrophobic compounds are extracted with a higher efficiency than less hydrophobic compounds analogous to bioaccumulation in organisms. In an initial screening the concentration of potential bioaccumulating substances (PBS) was measured in 20 effluent samples form various origins. The 5 samples with the highest PBS content were studied in detail in order to identify the dominant PBS compounds using SPME-GCMS. Between 35 and 60% of the total amount of PBS was identified using both AMDIS software and the NIST library as well as the RIZA GCMS database of substances found in Dutch rivers. Identified compounds include a.o. halogenated alkylbenzenes, phtalates and PAHs. The high fraction of identified PBS enables future abatement of the emission of PBS in process integrated and/or end-of-pipe strategies. Key words: bioaccumulation, Whole Effluent Assessment, Solid Phase Micro Extraction, GCMS |
