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(16-26) Chemotactic properties of polynuclear aromatic hydrocarbon bacteria isolated from coal tar and oil-polluted rhizospheres. Ortega - Calvo, José Julio*,1, Marchenko, A. I.2, Borobyev, A. V.2, Borovick, R. V.2, 1 Instituto de Recursos Naturales y Agrobiología, CSIC, Sevilla, Spain2 Research Centre for Toxicology and Hygienic Regulation of Biopreparations, Serpukhov, Russia ABSTRACT- The limited mass-transfer in PAH-contaminated soils during bioremediation treatments often causes difficulties for the achievement of regulatory decontamination end-points. This work constitutes a first attempt to evaluate chemotaxis as a bioavailability-promoting trait in PAH-degrading bacterial inoculants, for use in bioremediation either alone or together with phytoremediation. For this aim, strains capable to degrade different PAH (naphthalene, phenanthrene, anthracene, pyrene and fluoranthene) were isolated from rhizospheres contaminated with coal tar and oil. The chemotactic response towards different seed extracts (wheat, oat, pea, alfalfa, etc.) was quantified with a new densitometric method. The pathogenicity of the strains to warm-blooded animals was also tested according to high-safety levels, by determining virulence, dissemination, toxicity, and toxigenicity of the strains in outbred albino rats and mice. Those three strains showing no pathogenic activity were selected for detailed chemotaxis studies with PAH and seed extracts. Key words: Chemotactic, Rhizospheres |
