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(58-01) The Use of Biomarker Responses in Caged Carp for the Assessment of Inland Water Pollution. van der Oost, Ron*,1, Verweij, Frank1, van der Molen, Natascha, Satumalay, Karel1, Vermeulen, Nico2, 1 OMEGAM Environmental Research Laboratory, City of Amsterdam, Amsterdam, Netherlands2 Free University Amsterdam, Amsterdam, Netherlands ABSTRACT- The feasibility of using caged carp to assess the environmental quality of inland waters was investigated with genetically identical carp of a cultured F1 hybrid fish line. The primary objective of the experiments was to develop a reliable and reproducible method to monitor the inland water pollution and to classify the environmental quality of freshwater sites. From 1996 to 2001 several caging experiments have been carried out with carp (Cyprinus carpio) at several freshwater sites with varying levels of pollution in and around the City of Amsterdam. The data were used to validate freshwater quality assessment using fish biomarker responses under standardized conditions. Carp livers, bile and plasma were isolated in order to determine levels and activities of a suite of biochemical parameters: hepatic phase I enzymes, hepatic phase II enzymes and cofactors, hepatic antioxidant enzymes, biliary PAH metabolites, plasma transaminases, plasma vitellogenin levels, neuromuscular toxicity, DNA damage, etc. The strongest pollution-related effects were observed for the hepatic phase I enzymes (EROD and CYP1A) and PAH metabolites (1-OH pyrene), while less pronounced effects were observed for the phase II enzymes and cofactors (UDPGT, GST and GSH:GSSG). No clear differences in levels or activities of antioxidant enzymes, vitellogenin and serum transaminases were observed between carp caged at the polluted and the reference sites. The reproducibility of this method will be evaluated by comparing measurements in different years and seasons. Biomarker responses will be compared with chemical data in sediments and fish as well as from SPMDs ('semi permeable membrane devices'). A substantial part of the investigations was carried out with financial support of the City of Amsterdam and the European Commission ('CITY FISH' project). Key words: multiple biomarkers, caged carp, environmental risk assessment |
